Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 20 mM, activates | Gallus gallus | |
Nonidet P-40 | activates | Gallus gallus | |
Triton X-100 | 0.1%, 2fold stimulation | Gallus gallus | |
Urea | 2 mM, activates | Gallus gallus |
General Stability | Organism |
---|---|
highly labile enzyme, DTT stabilizes during purification, 0.25 M or 0.1 M galactose or glycerol 25% v/v do not stabilize | Gallus gallus |
unstable during purification | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Gallus gallus | |
AMP | - |
Gallus gallus | |
ATP | - |
Gallus gallus | |
CDP | - |
Gallus gallus | |
Co2+ | 6 mM: 50% inhibition | Gallus gallus | |
concanavalin A | 0.3 M methyl-alpha-D-glucopyranoside protects, but not galactose | Gallus gallus | |
Galactosylhydroxylysine | - |
Gallus gallus | |
GDP | - |
Gallus gallus | |
gelatinized calf skin collagen | above 60 mg/ml | Gallus gallus | |
human renal basement membrane collagen | glucose-free | Gallus gallus | |
hydroxylysine | weak | Gallus gallus | |
Mn2+ | above 2.5 mM | Gallus gallus | |
additional information | not inhibited by lysine, free monosaccharides, 1 mM lactose, 1 mM sucrose, 2 mM uridine | Gallus gallus | |
UDP | - |
Gallus gallus | |
UDPgalacturonate | - |
Gallus gallus | |
UDPglucose | - |
Gallus gallus | |
UDPglucuronate | - |
Gallus gallus | |
UMP | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km: 16 mg/ml, gelatinized calf skin collagen | Gallus gallus | |
0.02 | 0.023 | UDPgalactose | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum cisterna | presumed location within | Gallus gallus | - |
- |
membrane | associated with membrane structures | Gallus gallus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | requirement | Gallus gallus | |
Mn2+ | optimal concentration: 2-2.5 mM | Gallus gallus | |
additional information | not activated by Co2+ | Gallus gallus | |
additional information | not activated by Mg2+ | Gallus gallus | |
additional information | not activated by Fe2+ | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gallus gallus | collagen biosynthesis | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethylene glycol | 50% v/v, stable to | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
white Leghorn chicken | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | presence of carbohydrate units in enzyme molecule | Gallus gallus |
Purification (Comment) | Organism |
---|---|
250-300fold | Gallus gallus |
optimal extraction requires high concentrations of detergent and salt | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | 14-15 days old | Gallus gallus | - |
Storage Stability | Organism |
---|---|
-20°C, 50% v/v ethylene glycol, several weeks, stable | Gallus gallus |
4°C, unstable to storage, urea, galactose, Triton X-100, glycerol or ethylene glycol do not stabilize | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no acceptor: free hydroxylysine | Gallus gallus | ? | - |
? | |
additional information | collagen biosynthesis | Gallus gallus | ? | - |
? | |
UDPgalactose + procollagen 5-hydroxy-L-lysine | gelatinized insoluble calf skin collagen | Gallus gallus | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |